1.what is the desired gene in the diagram? 2. which is the vector of this gene of interest? 3.after the gene is inserted into the vector,what process is very important to insure that insulin is produced abundantly? 4.By means of a flow chart show the steps involve in the production of genetically engeenered human insulin by indicating the event that takes place in each step.
The desired gene may be microinjected directly into the cell or it may be inserted into the core of a virus by gene splicing and the virus allowed to infect the cell for replication of the gene.
The Scientist is clone their gene of interest into a Ti Plasmid, along with DNA sequences that make sure the gene can be expressed in plants. They then reintroduce the vector The gene for human insulin is inserted into the gap in the plasmid. This plasmid is now genetically modified. The genetically modified plasmid is introduced into a new bacteria or yeast cell. This cell then divides rapidly and starts making insulinto Agrobacterium cells and infect plants with the modified Agrobacterium, which delivers the vector to plant cells.
Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.
