Department of Education DIVISION OF NEGROS OCCIDENTAL Special Science Learning Toolkit No. 5 WORKSHEET 3
Write UP if the statement is Correct and DOWN if the statement is incorrect.
1. Wipe the outside of the containers, flasks, plates, and dishes with 70% salt solution before placing them in the cell culture hood.
2. Use sterile glass or disposable plastic pipettes and a pipettor to work with liquids, and use each pipette only many times to avoid cross contamination.
3. Always cap the bottles and flasks after use and seal multi-well plates with tape or place them in resealable bags to prevent microorganisms and airborne contaminants from gaining entry.
4. It is recommended to wear gloves. This will prevent any foreign contaminants from coming in contact with the person and sample during testing.
5. Always wipe your hands and work area with water and other solution.
6. Never cover a sterile flask, bottle, Petri dish, etc. until the instant you are ready to use it and never leave it open to the environment. Discard the cover as soon as you are finished.
7. Use only sterile glassware and other equipment.
8. Be careful not to talk, sing, or whistle while performing sterile procedures.
9. Perform experiments as rapidly as possible to minimize contamination.
10. Pour media and reagents directly from bottles or flasks.
3. A recipe calls for a oz. of sugar to make 7 cookies. Julianne decided to use only one-third of it to make it healthier. How much sugar did Julianne use? hoked 24 cupcakes,
