The Biuret reaction test is a type of analysis that determine the presence of the peptide bonds in a certain serum. This type of test depends on the number of peptide bonds that can be seen on the substance.
The reagent used for the Biuret Test are:
- Copper sulfate (CuSO40)
- Sodium hydroxide (NaOH)
- Sodium potássium tartarate
For the interpretation of data, a serum is said to be positive for the presence of peptide bonds if the solution change from color blue to deep purple.
