Below are the initial steps of manipulating the microscope. What is the correct sequence? 1. Place the specimen on the microscope stage and fix it with stage clips. 2. Then, adjust the mirror and diaphragm by looking through the eyepiece. 3. Slowly turn the coarse adjustment knob, until the image is focused 4. Turn the coarse adjustment knob to bring the objective lens close to the specimen. A. 1,2,3,4 B. 2,1,3,4 C. 1,4,2,3 D. 1, 4, 3, 2