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Plasmid method.
The most commonly used method of genetic engineering, the plasmid method uses small circular pieces of a DNA molecule to alter microorganisms, such as bacteria. The plasmid is placed in a container with enzymes that cut the plasmid up into small pieces
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This review highlights the most recent methods for genetic manipulation of A. baumannii including CRISPR based approaches, transposon mutagenesis, homologous recombination strategies, reporter systems and complementation techniques with the spotlight on those that can be applied to MDR clinical isolates.